Team:DTU-Denmark/Notebook/12 July 2013

From 2013.igem.org

(Difference between revisions)
(Amplification of cytochromes, AMO, HAO and Nir genes)
(Main purpose)
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*miniprep of biobrick transformants from [[Team:DTU-Denmark/Notebook/10_July_2013#Transformation_of_Biobricks|10-07-2013]]
*miniprep of biobrick transformants from [[Team:DTU-Denmark/Notebook/10_July_2013#Transformation_of_Biobricks|10-07-2013]]
*PCR with USER primers to amplify cytochromes, AMO, HAO  and Nir genes
*PCR with USER primers to amplify cytochromes, AMO, HAO  and Nir genes
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*gel analysis of PCR products from [[Team:DTU-Denmark/Notebook/11_July_2013#Combine_Nir_operon_with_pZA21|Nir operon combined with pZA21]] and [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| activity test of inducible AraBAD promoter]]
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*gel analysis of PCR products from [[Team:DTU-Denmark/Notebook/11_July_2013#Combine_Nir_operon_with_pZA21|Nir operon , pZA21]] and [[Team:DTU-Denmark/Notebook/11_July_2013#PCR_for_promoter_test| AraBAD promoter]]
==Who was in the lab==
==Who was in the lab==

Revision as of 15:20, 12 July 2013

Contents

208

Main purpose

Who was in the lab

Henrike, Julia, Kristian, Jakob, Gosia

Procedure

Amplification of cytochromes, AMO, HAO and Nir genes

Each PCR reaction was performed in triplicate.

Samples are named:

  • 1, 2, 3 -> cytochromes
  • 4, 5, 6 -> AMO
  • 7, 8, 9 -> HAO
  • 10, 11, 12 -> Nir