"

From 2013.igem.org

Contents 1 208 1.1 Main purpose 1.2 Who was in the lab 1.3 Procedure 1.3.1 Amplification of cytochromes, AMO, HAO and Nir genes

208

Main purpose

• miniprep of biobrick transformants from 10-07-2013
• PCR with USER primers to amplify cytochromes, AMO, HAO and Nir genes
• gel analysis of PCR products from Nir operon, AraBAD promoter and RFP
• USER reaction of RFP and pZA21 (with native promoter) and transformation of E. coli cells

Who was in the lab

Henrike, Julia, Kristian, Jakob, Gosia

Procedure

Amplification of cytochromes, AMO, HAO and Nir genes

Each PCR reaction was performed in triplicate.

Samples are named:

• 1, 2, 3 -> cytochromes
• 4, 5, 6 -> AMO
• 7, 8, 9 -> HAO
• 10, 11, 12 -> Nir