Team:DTU-Denmark/Notebook/13 August 2013

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13 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Kristian, Henrike, Gosia

Procedure


PCR (promoter library)

PCR to amplify USER fragments of the constitutive SPL, the constitutive reference promoter and the arabinose reference promoter.

Used 5% DMSO, 2 uL of mM MgCl2 and GC-buffer.

constitutive SPL

  • template: pZA21::RFP
  • primers: 52a, 52b1

constitutive reference

  • template: pZA21::RFP
  • primers: 52a, 52b2

arabinose reference

  • template:
  • primers: 51a,51b1

program

Results


Each gel is representing one row 17-07-2013; from left to right and up to down is row A to H:

temperature time cycles
98C 2:00 -
98C 2:00 -
98C 2:00 -
98C 2:00 -
98C 2:00 -

Conclusion



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