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Team:DTU-Denmark/Notebook/14 August 2013
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PCR reaction for amplification of Nir2 extraction fragment in order to obtain a huge amount to use as template to make a screening PCR for the Nir2 USER fragment | PCR reaction for amplification of Nir2 extraction fragment in order to obtain a huge amount to use as template to make a screening PCR for the Nir2 USER fragment | ||
| - | + | primers: | |
| + | template: P. aeruginosa cells resuspended in 100 uL | ||
| + | program: touchdown PCR (called U on Eppendorf) | ||
===Gel purification=== | ===Gel purification=== | ||
Revision as of 09:46, 14 August 2013
14 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Gosia, Julia, Henrike
Procedure
PCR
PCR reaction for amplification of Nir2 extraction fragment in order to obtain a huge amount to use as template to make a screening PCR for the Nir2 USER fragment
primers: template: P. aeruginosa cells resuspended in 100 uL program: touchdown PCR (called U on Eppendorf)
Gel purification
gel purify PCR products from constitutive promoter SPL and constitutive promoter ref
Results
Conclusion
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