"
Team:DTU-Denmark/Notebook/14 August 2013
From 2013.igem.org
(Difference between revisions)
(→PCR) |
(→Main purpose) |
||
| Line 5: | Line 5: | ||
==Main purpose== | ==Main purpose== | ||
<hr/> | <hr/> | ||
| + | * PCR for extraction of Nir2 from PAO1 | ||
==Who was in the lab== | ==Who was in the lab== | ||
Revision as of 11:42, 14 August 2013
14 August 2013
Contents |
lab 208
Main purpose
- PCR for extraction of Nir2 from PAO1
Who was in the lab
Gosia, Julia, Henrike
Procedure
PCR
PCR reaction for amplification of Nir2 extraction fragment in order to obtain a huge amount to use as template to make a screening PCR for the Nir2 USER fragment
primers: template: P. aeruginosa cells resuspended in 100 uL program: touchdown PCR (called U on Eppendorf)
Gel purification
gel purify PCR products from constitutive promoter SPL and constitutive promoter ref
Results
Conclusion
Navigate to the Previous or the Next Entry
