Team:DTU-Denmark/Notebook/15 August 2013

From 2013.igem.org

(Difference between revisions)
(Results)
(Results)
Line 35: Line 35:
* Nir2 USER touchdown negative
* Nir2 USER touchdown negative
* 1 kb ladder
* 1 kb ladder
 +
 +
[[File:2013-08-15 nir2 user.jpg|600px]]
 +
 +
The purification has the wrong length for Nir2 and was therefore discarded.
==Conclusion==
==Conclusion==

Revision as of 14:13, 15 August 2013

15 August 2013

Navigate to the Previous or the Next Entry

Contents

lab 208


Main purpose


  • Plasmid isolation of CycAX, TAT3-2, TAT3-1a, Sec2, HAO, pZA21::RFP and pZA21::araBAD::RFP.

Who was in the lab


Ariadni, Helen, Kristian, Julia

Procedure


Plasmid isolation

Plasmid have been isolated following the protocol provided by ORIGENE PowerPrep HP Plasmid Miniprep Kit.

Glycerol stock cultures

-80C stock solution of Sec2, TAT3-1a, TAT3-2

Results


  • 1 kb ladder
  • purification of the extraction fragment of Nir2
  • Nir2 USER touchdown, sample 1
  • Nir2 USER touchdown, sample 2
  • Nir2 USER touchdown, sample 3
  • Nir2 USER touchdown, sample 4
  • Nir2 USER touchdown, sample 5
  • Nir2 USER touchdown negative
  • 1 kb ladder

2013-08-15 nir2 user.jpg

The purification has the wrong length for Nir2 and was therefore discarded.

Conclusion


Navigate to the Previous or the Next Entry