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Team:DTU-Denmark/Notebook/15 August 2013
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* 1 kb ladder | * 1 kb ladder | ||
* purification of the extraction fragment of Nir2 | * purification of the extraction fragment of Nir2 | ||
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The purification has the wrong length for Nir2 and was therefore discarded. | The purification has the wrong length for Nir2 and was therefore discarded. | ||
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| + | ===transformation=== | ||
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| + | Yesterday's transformation yielded too many colonies that were inseparable, it will therefore be repeated and less volume will be plated. | ||
==Conclusion== | ==Conclusion== | ||
Revision as of 14:16, 15 August 2013
15 August 2013
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Contents |
lab 208
Main purpose
- Plasmid isolation of CycAX, TAT3-2, TAT3-1a, Sec2, HAO, pZA21::RFP and pZA21::araBAD::RFP.
Who was in the lab
Ariadni, Helen, Kristian, Julia
Procedure
Plasmid isolation
Plasmid have been isolated following the protocol provided by ORIGENE PowerPrep HP Plasmid Miniprep Kit.
Glycerol stock cultures
-80C stock solution of Sec2, TAT3-1a, TAT3-2
Results
gel
- 1 kb ladder
- purification of the extraction fragment of Nir2
- Nir2 USER touchdown, sample 1
- Nir2 USER touchdown, sample 2
- Nir2 USER touchdown, sample 3
- Nir2 USER touchdown, sample 4
- Nir2 USER touchdown, sample 5
- Nir2 USER touchdown negative
- 1 kb ladder
The purification has the wrong length for Nir2 and was therefore discarded.
transformation
Yesterday's transformation yielded too many colonies that were inseparable, it will therefore be repeated and less volume will be plated.
Conclusion
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