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lab 208

Main purpose

• Plasmid isolation of CycAX, TAT3-2, TAT3-1a, Sec2, HAO, pZA21::RFP and pZA21::araBAD::RFP.

Who was in the lab

Ariadni, Helen, Kristian, Julia

Procedure

Plasmid isolation

Plasmid have been isolated following the protocol provided by ORIGENE PowerPrep HP Plasmid Miniprep Kit.

Glycerol stock cultures

-80C stock solution of Sec2, TAT3-1a, TAT3-2

Results

• 1 kb ladder
• purification of the extraction fragment of Nir2
• Nir2 USER touchdown, sample 1
• Nir2 USER touchdown, sample 2
• Nir2 USER touchdown, sample 3
• Nir2 USER touchdown, sample 4
• Nir2 USER touchdown, sample 5
• Nir2 USER touchdown negative
• 1 kb ladder

The purification has the wrong length for Nir2 and was therefore discarded.

Conclusion

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