Team:DTU-Denmark/Notebook/15 August 2013

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15 August 2013

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Contents

lab 208


Main purpose


  • Plasmid isolation of CycAX, TAT3-2, TAT3-1a, Sec2, HAO, pZA21::RFP and pZA21::araBAD::RFP.

Who was in the lab


Ariadni, Helen, Kristian, Julia

Procedure


Plasmid isolation

Plasmid have been isolated following the protocol provided by ORIGENE PowerPrep HP Plasmid Miniprep Kit.

Glycerol stock cultures

-80C stock solution of Sec2, TAT3-1a, TAT3-2

Results


gel

  • 1 kb ladder
  • purification of the extraction fragment of Nir2
  • Nir2 USER touchdown, sample 1
  • Nir2 USER touchdown, sample 2
  • Nir2 USER touchdown, sample 3
  • Nir2 USER touchdown, sample 4
  • Nir2 USER touchdown, sample 5
  • Nir2 USER touchdown negative
  • 1 kb ladder

2013-08-15 nir2 user.jpg

The purification has the wrong length for Nir2 and was therefore discarded.

transformation

Yesterday's transformation yielded too many colonies that were inseparable, it will therefore be repeated and less volume will be plated.

Conclusion


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