Team:DTU-Denmark/Notebook/16 August 2013

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16 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Kristian, Julia, Henrike

Procedure


Gradient PCR for Nir2 USER

Set up new gradient PCR for Nir2 USER to run in 223. Reaction mix without MgCl2 but using DMSO and GC-buffer.

template: Nir2 extraction fragment

primers: 40a, 40b

additives: 5% DMSO

buffer: GC

Gradient went from 60C to 72C in 12 steps.

sample # 1 2 3 4 5 6 7 8 9 10 11 12
temperature 60.2 60.5 61.6 63.0 64.2 65.4 66.6 67.6 68.9 70.2 71.6 72

Results


Conclusion


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