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Team:DTU-Denmark/Notebook/19 August 2013
From 2013.igem.org
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==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
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| + | ===Preparation of constructs to send for sequencing=== | ||
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| + | Julia, please fill in :) | ||
| + | |||
| + | ===Gradient PCR for creation of Biobricks=== | ||
| + | |||
| + | Made a screening to find good conditions for the extraction of our constructs for subsequent ligation into BioBricks. | ||
| + | |||
| + | ===PCR to linearize pZA21::RFP::araBAD=== | ||
| + | |||
| + | ===colony PCR on one of the Hello Wolrd constructs to confirm it's intact=== | ||
| + | |||
| + | Performed colony PCR on the construct containing Sec2 to check the template is correct because our PCRs on it are not working. | ||
==Results== | ==Results== | ||
Revision as of 19:08, 19 August 2013
19 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Kristian, Henrike, Julia
Procedure
Preparation of constructs to send for sequencing
Julia, please fill in :)
Gradient PCR for creation of Biobricks
Made a screening to find good conditions for the extraction of our constructs for subsequent ligation into BioBricks.
PCR to linearize pZA21::RFP::araBAD
colony PCR on one of the Hello Wolrd constructs to confirm it's intact
Performed colony PCR on the construct containing Sec2 to check the template is correct because our PCRs on it are not working.
Results
Conclusion
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