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Team:DTU-Denmark/Notebook/1 August 2013
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==Procedure== | ==Procedure== | ||
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| + | ===gel purification=== | ||
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| + | Gel purified HAO fragment for USER cloning and pZA21 with endings for cloning with the 2-part Nir. Used QIAEX kit (without columns). | ||
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| + | ===PCR=== | ||
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| + | Set up PCR to amplify AMO as fragment for USER cloning. Run triplicates on 50C and 3:00 and dublicates on a ramp program 56C -> 50C with 0.1C/s and 3:00 | ||
==Results== | ==Results== | ||
Revision as of 16:33, 1 August 2013
1 August 2013
Contents |
lab ...
Main purpose
Who was in the lab
Procedure
gel purification
Gel purified HAO fragment for USER cloning and pZA21 with endings for cloning with the 2-part Nir. Used QIAEX kit (without columns).
PCR
Set up PCR to amplify AMO as fragment for USER cloning. Run triplicates on 50C and 3:00 and dublicates on a ramp program 56C -> 50C with 0.1C/s and 3:00
Results
Gels
1% gel for PCR of cytochromes with His-tag
- neg
- cycAX with His-tag at 45C
- cycAX with His-tag at 45C
- cycAX with His-tag at 50C
- cycAX with His-tag at 50C
- 1 kb ladder
1% gel for PCR products of Nir
- 1 kb ladder
- Nir part 2
- Nir part 2
- Nir part 1
- Nir part 1
- neg
Conclusion
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