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Team:DTU-Denmark/Notebook/21 August 2013
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{{:Team:DTU-Denmark/Templates/StartPage|21 August 2013}} | {{:Team:DTU-Denmark/Templates/StartPage|21 August 2013}} | ||
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Navigate to the [[Team:DTU-Denmark/Notebook/20_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/22_August_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/20_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/22_August_2013|Next]] Entry | ||
=lab 208= | =lab 208= | ||
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==Main purpose== | ==Main purpose== | ||
<hr/> | <hr/> | ||
| + | *USER reaction | ||
| + | *running gels | ||
==Who was in the lab== | ==Who was in the lab== | ||
| Line 16: | Line 17: | ||
===USER ligation and transformation=== | ===USER ligation and transformation=== | ||
| - | with opened pZA21::ara and HAO, AMO, Cyc | + | *with opened pZA21::ara and HAO, AMO, Cyc |
| - | also Nir1 + Nir2 in | + | *also tried to insert Nir1 + Nir2 in pZA21 with the fitting ends |
==Results== | ==Results== | ||
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* 1 kb ladder | * 1 kb ladder | ||
| - | + | [[File:2013-08-21 big screen.jpg|600px]] | |
* 1 kb ladder | * 1 kb ladder | ||
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* 1 kb ladder | * 1 kb ladder | ||
| - | + | [[File:2013-08-21 small screen.jpg|600px]] | |
* 1 kb ladder | * 1 kb ladder | ||
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* screening sample C12 | * screening sample C12 | ||
| - | + | [[File:2013-08-21 small screen 2.jpg|600px]] | |
==Conclusion== | ==Conclusion== | ||
<hr/> | <hr/> | ||
| + | |||
| + | PCR: | ||
| + | *got pZA21::RFP:araBAD linearized and Sec2 | ||
| + | *the gradient PCR did not work | ||
| + | |||
Navigate to the [[Team:DTU-Denmark/Notebook/20_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/22_August_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/20_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/22_August_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 17:48, 28 September 2013
21 August 2013
Contents |
lab 208
Main purpose
- USER reaction
- running gels
Who was in the lab
Kristian, Henrike
Procedure
USER ligation and transformation
- with opened pZA21::ara and HAO, AMO, Cyc
- also tried to insert Nir1 + Nir2 in pZA21 with the fitting ends
Results
Gels
- 1 kb ladder
- pZA21::RFP:araBAD linearized
- pZA21::RFP:araBAD linearized (duplicate)
- test PCR Sec2
- test PCR Sec2 (duplicate)
- screening sample A4
- screening sample A5
- screening sample A6
- screening sample A7
- screening sample A8
- screening sample A9
- screening sample A10
- screening sample A11
- screening sample A12
- screening sample B1
- screening sample B2
- screening sample B3
- screening sample B4
- 1 kb ladder
- 1 kb ladder
- screening sample B5
- screening sample B6
- screening sample B7
- screening sample B8
- screening sample B9
- screening sample B10
- screening sample B11
- screening sample B12
- screening sample C1
- screening sample C2
- screening sample C3
- screening sample C4
- screening sample C5
- 1 kb ladder
- 1 kb ladder
- screening sample C6
- screening sample C7
- screening sample C8
- screening sample C9
- screening sample C10
- screening sample C11
- screening sample C12
Conclusion
PCR:
- got pZA21::RFP:araBAD linearized and Sec2
- the gradient PCR did not work
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