lab 208

Main purpose

• USER reaction
• running gels

Who was in the lab

Kristian, Henrike

Procedure

USER ligation and transformation

• with opened pZA21::ara and HAO, AMO, Cyc

• also tried to insert Nir1 + Nir2 in pZA21 with the fitting ends

Results

Gels

• 1 kb ladder
• pZA21::RFP:araBAD linearized
• pZA21::RFP:araBAD linearized (duplicate)
• test PCR Sec2
• test PCR Sec2 (duplicate)
• screening sample A4
• screening sample A5
• screening sample A6
• screening sample A7
• screening sample A8
• screening sample A9
• screening sample A10
• screening sample A11
• screening sample A12
• screening sample B1
• screening sample B2
• screening sample B3
• screening sample B4
• 1 kb ladder

• 1 kb ladder
• screening sample B5
• screening sample B6
• screening sample B7
• screening sample B8
• screening sample B9
• screening sample B10
• screening sample B11
• screening sample B12
• screening sample C1
• screening sample C2
• screening sample C3
• screening sample C4
• screening sample C5
• 1 kb ladder

• 1 kb ladder
• screening sample C6
• screening sample C7
• screening sample C8
• screening sample C9
• screening sample C10
• screening sample C11
• screening sample C12

Conclusion

PCR:

• got pZA21::RFP:araBAD linearized and Sec2
• the gradient PCR did not work

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