Team:DTU-Denmark/Notebook/22 August 2013

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template: Sec2 miniprep
template: Sec2 miniprep
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program:
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{| class="wikitable" style="text-align: right"
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! temperature !! time !! cycles
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|-
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|  98C || 2:00 || -
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|-
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|  98C || 0:10 || 10
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|-
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|  63C || 1:00 || 10
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|-
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| -0.5C per cycle
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|-
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|  72C || 1:00 || 10
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|-
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|  98C || 0:10 || 25
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|-
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|  53C || 1:00 || 25
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|-
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|  72C || 1:00 || 25
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|-
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|  72C || 5:00 || -
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|-
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|  10C || hold || -
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|-
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|}
==Results==
==Results==

Revision as of 12:35, 22 August 2013

22 August 2013

Contents

lab 208

Main purpose

Who was in the lab

Kristian, Henrike

Procedure

USER ligation and transformation

Redid for HAO and cyc in arabinose inducible pZA21::araBAD and for Nir fragments

PCR for Biobrick parts

Set up a new PCR reaction for Biobrick parts using HF buffer and 5% DMSO but no MgCl2. PCR was run on a touchdown program

primers: 53a, 53b

template: Sec2 miniprep

program:

temperature time cycles
98C 2:00 -
98C 0:10 10
63C 1:00 10
-0.5C per cycle
72C 1:00 10
98C 0:10 25
53C 1:00 25
72C 1:00 25
72C 5:00 -
10C hold -

Results

Conclusion

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