lab 208

Main purpose

Who was in the lab

Kristian, Henrike

Procedure

PCR Biobrick Isolation

Used new primers to isolate Hello World constructs. Used HF buffer and tested three different concentrations of DMSO: 0%, 2% and 5%.

Primers: 56a, 56b

template: TAT2-1 (miniprep)

Results

Gel electrophoresis

• 1 kb ladder
• Sec2 Biobrick
• Sec2 Biobrick
• Sec2 neg
• col AMO 1
• col AMO 2
• col AMO 3
• col AMO 4
• col AMO 5
• col AMO 6
• col AMO 7
• col AMO 8
• col AMO 9
• col AMO 10
• 1 kb ladder

• 1 kb ladder
• col Nir
• constitutive reference promoter
• constitutive reference promoter
• Biobrick Isolation TAT2-1 negative
• Biobrick Isolation TAT2-1 2% DMSO
• Biobrick Isolation TAT2-1 5% DMSO
• Biobrick Isolation TAT2-1 w/o DMSO
• 1 kb ladder

Conclusion

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