Team:DTU-Denmark/Notebook/23 July 2013

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(Difference between revisions)
(Created page with "===Results=== ==Verification gel== 1% gel to verify our earlier PCR purifications wells (in bracets position of the purification in the gene box): *1: 1kb plus ladder *2: HAO...")
(Verification gel)
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*7: Nir extracted from P. aeruginosa with flanking primers (B8)
*7: Nir extracted from P. aeruginosa with flanking primers (B8)
*8: Nir extracted from P. aeruginosa with flanking primers (B9)
*8: Nir extracted from P. aeruginosa with flanking primers (B9)
 +
*9: pZA21 USER fragment, DpnI treated (F1)
 +
*10: cycAX USER fragment (G1)
 +
*11: AMO USER fragment (G2)
 +
*12: HAO USER fragment (G3)
 +
*13: Nir USER fragment (G4)
 +
*14: AMO USER fragment (G5)
 +
*15: 1kb plus ladder

Revision as of 09:26, 24 July 2013

Results

Verification gel

1% gel to verify our earlier PCR purifications

wells (in bracets position of the purification in the gene box):

  • 1: 1kb plus ladder
  • 2: HAO extracted from N. europeae with flanking primers (A4)
  • 3: AMO extracted from N. europeae with flanking primers (A6)
  • 4: cycAX extracted from N. europeae with flanking primers (A5)
  • 5: Nir extracted from P. aeruginosa with flanking primers (A7)
  • 6: Nir extracted from P. aeruginosa with flanking primers (A8)
  • 7: Nir extracted from P. aeruginosa with flanking primers (B8)
  • 8: Nir extracted from P. aeruginosa with flanking primers (B9)
  • 9: pZA21 USER fragment, DpnI treated (F1)
  • 10: cycAX USER fragment (G1)
  • 11: AMO USER fragment (G2)
  • 12: HAO USER fragment (G3)
  • 13: Nir USER fragment (G4)
  • 14: AMO USER fragment (G5)
  • 15: 1kb plus ladder