"
Team:DTU-Denmark/Notebook/23 July 2013
From 2013.igem.org
(Difference between revisions)
(→Verification gel) |
(→Verification gel) |
||
| Line 22: | Line 22: | ||
*14: AMO USER fragment (G5) | *14: AMO USER fragment (G5) | ||
*15: 1kb plus ladder | *15: 1kb plus ladder | ||
| + | |||
| + | |||
| + | ==colony PCR gel== | ||
| + | |||
| + | [[File:2013-07-23 colony cyc rfp.jpg|600px]] | ||
Revision as of 09:27, 24 July 2013
Results
Verification gel
1% gel to verify our earlier PCR purifications
wells (in bracets position of the purification in the gene box):
- 1: 1kb plus ladder
- 2: HAO extracted from N. europeae with flanking primers (A4)
- 3: AMO extracted from N. europeae with flanking primers (A6)
- 4: cycAX extracted from N. europeae with flanking primers (A5)
- 5: Nir extracted from P. aeruginosa with flanking primers (A7)
- 6: Nir extracted from P. aeruginosa with flanking primers (A8)
- 7: Nir extracted from P. aeruginosa with flanking primers (B8)
- 8: Nir extracted from P. aeruginosa with flanking primers (B9)
- 9: pZA21 USER fragment, DpnI treated (F1)
- 10: cycAX USER fragment (G1)
- 11: AMO USER fragment (G2)
- 12: HAO USER fragment (G3)
- 13: Nir USER fragment (G4)
- 14: AMO USER fragment (G5)
- 15: 1kb plus ladder
colony PCR gel
