Team:DTU-Denmark/Notebook/24 August 2013

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24 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Kristian, Henrike

Procedure


Results


Gels

  • 1 kb
  • colony PCR NirG
  • constitutive reference promoter, GC buffer
  • constitutive reference promoter, GC buffer + 3% DMSO
  • constitutive reference promoter, HF buffer
  • constitutive reference promoter, HF buffer + 3% DMSO
  • 1 kb

2013-08-24 nircol ref const.jpg

Gel could not be read and will be redone.

  • 1 kb
  • colony PCR cycAX, colony 2
  • colony PCR cycAX, colony 3
  • colony PCR cycAX, colony 4
  • colony PCR cycAX, colony 6
  • colony PCR cycAX, colony 7
  • colony PCR cycAX, colony 8
  • colony PCR cycAX, colony 9
  • constitutive reference promoter, GC buffer
  • constitutive reference promoter, GC buffer + 3% DMSO
  • constitutive reference promoter, HF buffer
  • constitutive reference promoter, HF buffer + 3% DMSO
  • 1 kb

2013-08-24 cyccol refconst.jpg

The insert is confirmed for the colonies 3, 4 and 8.

Conclusion


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