Team:DTU-Denmark/Notebook/25 August 2013
From 2013.igem.org
(Difference between revisions)
(→Colony PCR) |
(→Colony PCR) |
||
Line 14: | Line 14: | ||
===Colony PCR=== | ===Colony PCR=== | ||
+ | |||
+ | Performed colony PCR to confirm insert for HAO, AMO, cycAX and Nir transformants. Used Q5 premix with the following reaction mix: | ||
+ | |||
+ | {| class="wikitable" style="text-align: right" | ||
+ | ! compound !! amount | ||
+ | |- | ||
+ | | Q5 mix || 25 uL | ||
+ | |- | ||
+ | | FW primer || 3 uL | ||
+ | |- | ||
+ | | RV primer || 3 uL | ||
+ | |- | ||
+ | | template || 1 uL | ||
+ | |- | ||
+ | | MilliQ || 18 uL | ||
+ | |||
+ | Template was made by resuspending 1 culture in 100uL MilliQ. | ||
program: | program: | ||
Line 32: | Line 49: | ||
|- | |- | ||
|} | |} | ||
+ | |||
+ | annealing temperatures: | ||
+ | * cycAX | ||
+ | * | ||
+ | * | ||
+ | * | ||
==Results== | ==Results== |
Revision as of 14:00, 26 August 2013
25 August 2013
Navigate to the Previous or the Next Entry
Contents |
Lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR
Performed colony PCR to confirm insert for HAO, AMO, cycAX and Nir transformants. Used Q5 premix with the following reaction mix:
compound | amount | |||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Q5 mix | 25 uL | |||||||||||||||||||||
FW primer | 3 uL | |||||||||||||||||||||
RV primer | 3 uL | |||||||||||||||||||||
template | 1 uL | |||||||||||||||||||||
MilliQ | 18 uL
Template was made by resuspending 1 culture in 100uL MilliQ. program:
annealing temperatures:
ResultsConclusionNavigate to the Previous or the Next Entry
|