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Team:DTU-Denmark/Notebook/25 August 2013
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Revision as of 14:01, 26 August 2013
25 August 2013
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Contents |
Lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR
Performed colony PCR to confirm insert for HAO, AMO, cycAX and Nir transformants. Used Q5 premix with the following reaction mix:
| compound | amount |
|---|---|
| Q5 mix | 25 uL |
| FW primer | 3 uL |
| RV primer | 3 uL |
| template | 1 uL |
| MilliQ | 18 uL |
Template was made by resuspending 1 culture in 100uL MilliQ.
program:
| temperature | time | cycles |
|---|---|---|
| 98C | 10:00 | - |
| 98C | 0:10 | 36 |
| annealing temperature | 0:30 | 36 |
| 72C | 0:20 | 36 |
| 72C | 5:00 | - |
| 10C | hold | - |
annealing temperatures:
- cycAX
Results
Conclusion
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