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Revision as of 10:49, 25 July 2013 (view source) Biotacg (Talk | contribs) (→Main purpose) ← Older edit		Revision as of 10:49, 25 July 2013 (view source) Biotacg (Talk | contribs) (→Who was in the lab) Newer edit →
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Revision as of 10:49, 25 July 2013

Contents 1 208 1.1 Main purpose 1.2 Who was in the lab 1.3 Procedure 1.3.1 PCR in order to amplify AMO and HAO with USER primers 1.3.2 gel electrophoresis 1.4 Results 1.5 Conclusion

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Main purpose

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• PCR of AMO and HAO using USER primers and as a template using AMO and HAO extracted from chromosomal DNA with non-uracil primers
• verification of PCRs
• ON cultures and re-plating of Nir USER transformants from Colony PCR

Who was in the lab

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Kristian, Gosia, Julia

Procedure

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PCR in order to amplify AMO and HAO with USER primers

gel electrophoresis

1% agarose gel was loaded with samples as follows:

1. 1 kb ladder
2. AMO 5uL template, 1
3. AMO 5uL template, 2
4. AMO 10uL template, 1
5. AMO 10uL template, 2
6. HAO 5uL template, 1
7. HAO 5uL template, 2
8. HAO 10uL template, 1
9. HAO 10uL template, 2
10. restriction analysis cyc EcoRI
11. restriction analysis cyc HindIII
12. 1 kb ladder

Results

Conclusion

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