Team:DTU-Denmark/Notebook/26 August 2013

From 2013.igem.org

(Difference between revisions)
(Lab 208)
 
Line 18: Line 18:
Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies.
Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies.
-
 
-
==Results==
 
-
<hr/>
 
-
 
-
==Conclusion==
 
-
<hr/>
 
Navigate to the [[Team:DTU-Denmark/Notebook/25_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_August_2013|Next]] Entry
Navigate to the [[Team:DTU-Denmark/Notebook/25_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_August_2013|Next]] Entry
{{:Team:DTU-Denmark/Templates/EndPage}}
{{:Team:DTU-Denmark/Templates/EndPage}}

Latest revision as of 12:05, 4 October 2013

26 August 2013

Navigate to the Previous or the Next Entry

Contents

Lab 208


Main purpose


  • Replating of TAT3-1a

Who was in the lab


Henrike, Helen, Ariadni, Kristian, Julia

Procedure


TAT3-1a replating

TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin.

Glucose plates (USER reaction)

Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies.

Navigate to the Previous or the Next Entry