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Team:DTU-Denmark/Notebook/26 August 2013
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Navigate to the [[Team:DTU-Denmark/Notebook/25_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_August_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/25_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_August_2013|Next]] Entry | ||
=Lab 208= | =Lab 208= | ||
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TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin. | TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin. | ||
| - | ===Glucose plates=== | + | ===Glucose plates (USER reaction)=== |
Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies. | Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies. | ||
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Navigate to the [[Team:DTU-Denmark/Notebook/25_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_August_2013|Next]] Entry | Navigate to the [[Team:DTU-Denmark/Notebook/25_August_2013|Previous]] or the [[Team:DTU-Denmark/Notebook/27_August_2013|Next]] Entry | ||
{{:Team:DTU-Denmark/Templates/EndPage}} | {{:Team:DTU-Denmark/Templates/EndPage}} | ||
Latest revision as of 12:05, 4 October 2013
26 August 2013
Contents |
Lab 208
Main purpose
- Replating of TAT3-1a
Who was in the lab
Henrike, Helen, Ariadni, Kristian, Julia
Procedure
TAT3-1a replating
TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin.
Glucose plates (USER reaction)
Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies.
Navigate to the Previous or the Next Entry
