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Team:DTU-Denmark/Notebook/26 August 2013
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===TAT3-1a replating=== | ===TAT3-1a replating=== | ||
TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin. | TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin. | ||
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| + | ===Glucose plates=== | ||
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| + | Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies. | ||
==Results== | ==Results== | ||
Revision as of 14:12, 26 August 2013
26 August 2013
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Contents |
Lab 208
Main purpose
- Replating of TAT3-1a
Who was in the lab
Henrike, Helen, Ariadni, Kristian, Julia
Procedure
TAT3-1a replating
TAT3-1a construct transformants from glycerol stock (15-08-013) were replated on agarose plate with 40 ng/uL Kanamycin.
Glucose plates
Remade USER ligation and transformation of HAO and cycAX onto plates containing a surplus of glucose. This should reduce the leakiness of the araBAD promoter and improve the health of the transformants before induction so we can recover more colonies.
Results
Conclusion
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