Team:DTU-Denmark/Notebook/27 August 2013

From 2013.igem.org

(Difference between revisions)
(→Gel)
(→Procedure)
Line 11: Line 11:
==Procedure==
==Procedure==
<hr/>
<hr/>
 +
 +
===PCR reactions===
 +
 +
{| class="wikitable" style="text-align: right"
 +
! compound !! amount
 +
|  standard mix || additive mix
 +
|-
 +
|  Q5 mix || 25 uL
 +
|-
 +
|  FW primer || 3 uL
 +
|-
 +
|  RV primer || 3 uL
 +
|-
 +
|  template || 1 uL
 +
|-
 +
|  MilliQ || 18 uL
 +
|-
 +
|}
 +
 +
Template was made by resuspending 1 culture in 100uL MilliQ.
 +
 +
program:
 +
{| class="wikitable" style="text-align: right"
 +
! temperature !! time !! cycles
 +
|-
 +
|  98C || 10:00 || -
 +
|-
 +
|  98C || 0:10 || 36
 +
|-
 +
|  annealing temperature || 0:30 || 36
 +
|-
 +
|  72C || 0:20 || 36
 +
|-
 +
|  72C || 5:00 || -
 +
|-
 +
|  10C || hold || -
 +
|-
 +
|}
==Results==
==Results==

Revision as of 17:45, 27 August 2013

27 August 2013

Contents

lab 208

Main purpose

Who was in the lab

Procedure

PCR reactions

compound amount standard mix additive mix
Q5 mix 25 uL
FW primer 3 uL
RV primer 3 uL
template 1 uL
MilliQ 18 uL

Template was made by resuspending 1 culture in 100uL MilliQ.

program:

temperature time cycles
98C 10:00 -
98C 0:10 36
annealing temperature 0:30 36
72C 0:20 36
72C 5:00 -
10C hold -

Results

Gel

  • 1 kb ladder
  • pSB1C3 for Biobricks, HF buffer
  • pSB1C3 for Biobricks, HF buffer, 5%DMSO
  • pSB1C3 for Biobricks, GC buffer
  • pSB1C3 for Biobricks, GC buffer, 5%DMSO
  • negative pSB1C3 for Biobricks
  • pZA21::ara for USER ligation with Nir
  • pZA21::ara for USER ligation with Nir (duplicate)
  • 1 kb ladder

Note: It seems the ladder overflew.

2013-08-27 bb pzaarafornir.jpg

Conclusion

Navigate to the Previous or the Next Entry

Retrieved from "http://2013.igem.org/Team:DTU-Denmark/Notebook/27_August_2013"