Team:DTU-Denmark/Notebook/27 August 2013
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{| class="wikitable" style="text-align: right" | {| class="wikitable" style="text-align: right" | ||
! compound !! amount | ! compound !! amount | ||
- | | standard mix || additive mix | + | |- |
+ | | || standard mix || additive mix | ||
|- | |- | ||
| Q5 mix || 25 uL | | Q5 mix || 25 uL |
Revision as of 17:46, 27 August 2013
27 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
PCR reactions
compound | amount | |
---|---|---|
standard mix | additive mix | |
Q5 mix | 25 uL | |
FW primer | 3 uL | |
RV primer | 3 uL | |
template | 1 uL | |
MilliQ | 18 uL |
Template was made by resuspending 1 culture in 100uL MilliQ.
program:
temperature | time | cycles |
---|---|---|
98C | 10:00 | - |
98C | 0:10 | 36 |
annealing temperature | 0:30 | 36 |
72C | 0:20 | 36 |
72C | 5:00 | - |
10C | hold | - |
Results
Gel
- 1 kb ladder
- pSB1C3 for Biobricks, HF buffer
- pSB1C3 for Biobricks, HF buffer, 5%DMSO
- pSB1C3 for Biobricks, GC buffer
- pSB1C3 for Biobricks, GC buffer, 5%DMSO
- negative pSB1C3 for Biobricks
- pZA21::ara for USER ligation with Nir
- pZA21::ara for USER ligation with Nir (duplicate)
- 1 kb ladder
Note: It seems the ladder overflew.
Conclusion
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