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Team:DTU-Denmark/Notebook/27 August 2013
From 2013.igem.org
27 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
PCR reactions
| compound | amount | standard mix | additive mix |
|---|---|---|---|
| Q5 mix | 25 uL | ||
| FW primer | 3 uL | ||
| RV primer | 3 uL | ||
| template | 1 uL | ||
| MilliQ | 18 uL |
Template was made by resuspending 1 culture in 100uL MilliQ.
program:
| temperature | time | cycles |
|---|---|---|
| 98C | 10:00 | - |
| 98C | 0:10 | 36 |
| annealing temperature | 0:30 | 36 |
| 72C | 0:20 | 36 |
| 72C | 5:00 | - |
| 10C | hold | - |
Results
Gel
- 1 kb ladder
- pSB1C3 for Biobricks, HF buffer
- pSB1C3 for Biobricks, HF buffer, 5%DMSO
- pSB1C3 for Biobricks, GC buffer
- pSB1C3 for Biobricks, GC buffer, 5%DMSO
- negative pSB1C3 for Biobricks
- pZA21::ara for USER ligation with Nir
- pZA21::ara for USER ligation with Nir (duplicate)
- 1 kb ladder
Note: It seems the ladder overflew.
Conclusion
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