Team:DTU-Denmark/Notebook/27 July 2013

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27 July 2013

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Contents

Lab 208


Main purpose


Run gel on yesterdays PCR.

Who was in the lab


Kristian

Procedure


Gel casting and loading

Results


  • 1kb ladder
  • araBAD
  • araBAD
  • araBAD
  • Nir 1 min extension
  • Nir 1 min extension
  • pZA21::RFP no promoter
  • pZA21::RFP no promoter
  • pZA21::RFP no promoter
  • Negative control
  • Nir 1x
  • Nir 1x
  • Nir 5x
  • Nir 5x
  • 1kb ladder

Next line:

  • 1kb ladder
  • Nir 10x
  • Nir 10x
  • Nir 50x
  • Nir 50x
  • Nir 100x
  • Nir 100x

2013-07-26 ara Nir pZA21.jpg


  • 1kb ladder
  • Nir 500x
  • Nir 500x
  • Nir 2022
  • Nir 2022
  • Nir 2023
  • Nir 2023
  • Nir 2024
  • Nir 2024
  • Nir 2025
  • Nir 2025

2013-07-26 Nir new colony.jpg


Conclusion


PCR is obviously not working too well for us right now. Tomorrow all reagents will be chanced and some of the PCR's will be run again.

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