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Team:DTU-Denmark/Notebook/28 August 2013
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==Procedure== | ==Procedure== | ||
<hr/> | <hr/> | ||
| + | A gradient PCR was made for pSB1C3 and pZA21::ara for Nir | ||
| + | The table shows the composition of each reaction: | ||
| + | {| class="wikitable" style="text-align: right" | ||
| + | ! component !! 1 !! 2 !! 3 !! Neg | ||
| + | |- | ||
| + | | dNTPs || 1uL || 1uL || 1uL || 1uL | ||
| + | |- | ||
| + | | HF buffer || 10uL || 10uL || 10uL || 10uL | ||
| + | |- | ||
| + | | X7 polymerase || 0.5uL || 0.5uL || 0.5uL || 0.5uL | ||
| + | |- | ||
| + | | MilliQ water || 28.5uL || 28.5uL || 28.5uL || 29.5uL | ||
| + | |- | ||
| + | | template pSB1C3 || 1uL || 1uL || 1uL || - | ||
| + | |- | ||
| + | | FW primer 57a2 || 3uL || 3uL || 3uL || 3uL | ||
| + | |- | ||
| + | | RV primer 57b || 3uL || 3uL || 3uL || 3uL | ||
| + | |- | ||
| + | | DMSO (100%) || 2uL (4%) || 2uL (4%) || 2uL (4%) || 2uL (4%) | ||
| + | |- | ||
| + | | MgCl2 (50mM) || 1uL (1mM) || 1uL (1mM) || 1uL (1mM) || 1uL (1mM) | ||
| + | |- | ||
| + | |} | ||
==Results== | ==Results== | ||
Revision as of 13:57, 29 August 2013
28 August 2013
Contents |
Lab 208
Main purpose
Who was in the lab
Henrike, Kristian, Julia
Procedure
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The table shows the composition of each reaction:
| component | 1 | 2 | 3 | Neg |
|---|---|---|---|---|
| dNTPs | 1uL | 1uL | 1uL | 1uL |
| HF buffer | 10uL | 10uL | 10uL | 10uL |
| X7 polymerase | 0.5uL | 0.5uL | 0.5uL | 0.5uL |
| MilliQ water | 28.5uL | 28.5uL | 28.5uL | 29.5uL |
| template pSB1C3 | 1uL | 1uL | 1uL | - |
| FW primer 57a2 | 3uL | 3uL | 3uL | 3uL |
| RV primer 57b | 3uL | 3uL | 3uL | 3uL |
| DMSO (100%) | 2uL (4%) | 2uL (4%) | 2uL (4%) | 2uL (4%) |
| MgCl2 (50mM) | 1uL (1mM) | 1uL (1mM) | 1uL (1mM) | 1uL (1mM) |
Results
Conclusion
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