Team:DTU-Denmark/Notebook/28 August 2013

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A gradient PCR was made for pSB1C3 and pZA21::ara for Nir
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir
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The table shows the composition of each reaction:
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The [[Team:DTU-Denmark/Methods/PCR-mix|master mix]] contains 2% of DMSO (1uL of 100%DMSO) and 1mM MgCl2 (1uL of 50mM MgCl2) for each reaction.
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{| class="wikitable" style="text-align: right"
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! component !! 1 !! 2 !! 3 !! Neg
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|-
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| dNTPs || 1uL || 1uL || 1uL || 1uL
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|-
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| HF buffer || 10uL || 10uL || 10uL || 10uL
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|-
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| X7 polymerase || 0.5uL || 0.5uL || 0.5uL || 0.5uL
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| MilliQ water || 28.5uL || 28.5uL || 28.5uL || 29.5uL
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|-
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| template pSB1C3 || 1uL || 1uL || 1uL || -
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|-
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| FW primer 57a2 || 3uL || 3uL || 3uL || 3uL
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| RV primer 57b || 3uL || 3uL || 3uL || 3uL
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| DMSO (100%) || 2uL (4%) || 2uL (4%) || 2uL (4%) || 2uL (4%)
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| MgCl2 (50mM) || 1uL (1mM) || 1uL (1mM) || 1uL (1mM) || 1uL (1mM)
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|}
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==Results==
==Results==

Revision as of 14:09, 29 August 2013

28 August 2013

Contents

Lab 208

Main purpose

Who was in the lab

Henrike, Kristian, Julia

Procedure

A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The master mix contains 2% of DMSO (1uL of 100%DMSO) and 1mM MgCl2 (1uL of 50mM MgCl2) for each reaction.

Results

Conclusion

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