Team:DTU-Denmark/Notebook/28 August 2013

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A gradient PCR was made for pSB1C3 and pZA21::ara for Nir
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir
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The [[Team:DTU-Denmark/Methods/PCR-mix|master mix]] contains 2% of DMSO (1uL of 100%DMSO) and 1mM MgCl2 (1uL of 50mM MgCl2) for each reaction.
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The [[Team:DTU-Denmark/Methods/PCR-mix|master mix]] contains:
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*CG buffer,
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*X7 polymerase,
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*2% of DMSO (1uL of 100%DMSO) per reaction
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*1mM MgCl2 (1uL of 50mM MgCl2) per reaction
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{| class="wikitable" style="text-align: right"
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! component !! 1 !! 2
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|-
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| Template || pSB1C3 || pZA21::ara
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|-
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| Primers || 57a + 57b || 58a + 58b
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|-
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|}
==Results==
==Results==

Revision as of 14:17, 29 August 2013

28 August 2013

Contents

Lab 208

Main purpose

Who was in the lab

Henrike, Kristian, Julia

Procedure

A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The master mix contains:

  • CG buffer,
  • X7 polymerase,
  • 2% of DMSO (1uL of 100%DMSO) per reaction
  • 1mM MgCl2 (1uL of 50mM MgCl2) per reaction
component 1 2
Template pSB1C3 pZA21::ara
Primers 57a + 57b 58a + 58b

Results

Conclusion

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