Team:DTU-Denmark/Notebook/28 August 2013
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A gradient PCR was made for pSB1C3 and pZA21::ara for Nir | A gradient PCR was made for pSB1C3 and pZA21::ara for Nir | ||
- | The [[Team:DTU-Denmark/Methods/PCR-mix|master mix]] contains 2% of DMSO (1uL of 100%DMSO) | + | The [[Team:DTU-Denmark/Methods/PCR-mix|master mix]] contains: |
+ | *CG buffer, | ||
+ | *X7 polymerase, | ||
+ | *2% of DMSO (1uL of 100%DMSO) per reaction | ||
+ | *1mM MgCl2 (1uL of 50mM MgCl2) per reaction | ||
+ | {| class="wikitable" style="text-align: right" | ||
+ | ! component !! 1 !! 2 | ||
+ | |- | ||
+ | | Template || pSB1C3 || pZA21::ara | ||
+ | |- | ||
+ | | Primers || 57a + 57b || 58a + 58b | ||
+ | |- | ||
+ | |} | ||
==Results== | ==Results== |
Revision as of 14:17, 29 August 2013
28 August 2013
Contents |
Lab 208
Main purpose
Who was in the lab
Henrike, Kristian, Julia
Procedure
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The master mix contains:
- CG buffer,
- X7 polymerase,
- 2% of DMSO (1uL of 100%DMSO) per reaction
- 1mM MgCl2 (1uL of 50mM MgCl2) per reaction
component | 1 | 2 |
---|---|---|
Template | pSB1C3 | pZA21::ara |
Primers | 57a + 57b | 58a + 58b |
Results
Conclusion
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