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Team:DTU-Denmark/Notebook/28 August 2013
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*1mM MgCl2 (1uL of 50mM MgCl2) per reaction | *1mM MgCl2 (1uL of 50mM MgCl2) per reaction | ||
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| - | ! component !! 1 !! 2 | + | ! component vs reaction !! 1 !! 2 |
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| Template || pSB1C3 || pZA21::ara | | Template || pSB1C3 || pZA21::ara | ||
Revision as of 14:17, 29 August 2013
28 August 2013
Contents |
Lab 208
Main purpose
Who was in the lab
Henrike, Kristian, Julia
Procedure
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The master mix contains:
- CG buffer,
- X7 polymerase,
- 2% of DMSO (1uL of 100%DMSO) per reaction
- 1mM MgCl2 (1uL of 50mM MgCl2) per reaction
| component vs reaction | 1 | 2 |
|---|---|---|
| Template | pSB1C3 | pZA21::ara |
| Primers | 57a + 57b | 58a + 58b |
Results
Conclusion
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