Team:DTU-Denmark/Notebook/28 August 2013
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Revision as of 14:21, 29 August 2013
28 August 2013
Contents |
Lab 208
Main purpose
Who was in the lab
Henrike, Kristian, Julia
Procedure
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The master mix contains:
- CG buffer,
- X7 polymerase,
- 2% of DMSO (1uL of 100%DMSO) per reaction
- 1mM MgCl2 (1uL of 50mM MgCl2) per reaction
1 | 2 | |
---|---|---|
Template | pSB1C3 | pZA21::ara |
Primers | 57a + 57b | 58a + 58b |
Temperature range | 58C-78C | 50C-65C |
Results
Conclusion
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