Team:DTU-Denmark/Notebook/28 August 2013

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(Procedure)
(Procedure)
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| Temperature range || 58C-78C || 50C-65C
| Temperature range || 58C-78C || 50C-65C
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| Annealing time per cycle || 1:30 min || 2 min
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| Elongation time per cycle || 1:30 min || 2 min
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Each reaction was run in one of the 12 different annealing temperatures within the range indicated in the table.
Each reaction was run in one of the 12 different annealing temperatures within the range indicated in the table.
 +
We followed the [[Team:DTU-Denmark/Methods/PCR#Generel_example_on_a_normal_PCR-program|standard PCR program]].
==Results==
==Results==

Revision as of 14:34, 29 August 2013

28 August 2013

Contents

Lab 208


Main purpose


Who was in the lab


Henrike, Kristian, Julia

Procedure


A gradient PCR was made for pSB1C3 and pZA21::ara for Nir.

The master mix contains:

  • CG buffer,
  • X7 polymerase,
  • 2% of DMSO (1uL of 100%DMSO) per reaction
  • 1mM MgCl2 (1uL of 50mM MgCl2) per reaction
1 2
Template pSB1C3 pZA21::ara
Primers 57a + 57b 58a + 58b
Temperature range 58C-78C 50C-65C
Elongation time per cycle 1:30 min 2 min

Each reaction was run in one of the 12 different annealing temperatures within the range indicated in the table. We followed the standard PCR program.

Results


Conclusion


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