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Team:DTU-Denmark/Notebook/28 August 2013
From 2013.igem.org
28 August 2013
Contents |
Lab 208
Main purpose
Who was in the lab
Henrike, Kristian, Julia
Procedure
A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The table shows the composition of each reaction:
| component | 1 | 2 | 3 | Neg |
|---|---|---|---|---|
| dNTPs | 1uL | 1uL | 1uL | 1uL |
| HF buffer | 10uL | 10uL | 10uL | 10uL |
| X7 polymerase | 0.5uL | 0.5uL | 0.5uL | 0.5uL |
| MilliQ water | 28.5uL | 28.5uL | 28.5uL | 29.5uL |
| template pSB1C3 | 1uL | 1uL | 1uL | - |
| FW primer 57a2 | 3uL | 3uL | 3uL | 3uL |
| RV primer 57b | 3uL | 3uL | 3uL | 3uL |
| DMSO (100%) | 2uL (4%) | 2uL (4%) | 2uL (4%) | 2uL (4%) |
| MgCl2 (50mM) | 1uL (1mM) | 1uL (1mM) | 1uL (1mM) | 1uL (1mM) |
Results
Conclusion
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