Team:DTU-Denmark/Notebook/28 August 2013

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28 August 2013

Contents

1 Lab 208

Lab 208

Main purpose

Who was in the lab

Henrike, Kristian, Julia

Procedure

A gradient PCR was made for pSB1C3 and pZA21::ara for Nir The master mix contains:

CG buffer,
X7 polymerase,
2% of DMSO (1uL of 100%DMSO) per reaction
1mM MgCl2 (1uL of 50mM MgCl2) per reaction

	1	2
Template	pSB1C3	pZA21::ara
Primers	57a + 57b	58a + 58b
Temperature range	58C-78C	50C-65C

Results

Conclusion

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