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Team:DTU-Denmark/Notebook/29 August 2013
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| - | =lab | + | =lab 208= |
<hr/> | <hr/> | ||
==Main purpose== | ==Main purpose== | ||
| Line 14: | Line 14: | ||
==Results== | ==Results== | ||
<hr/> | <hr/> | ||
| + | |||
| + | ===Gel pictures=== | ||
| + | |||
| + | Ran gels on yesterday's PCRs to create psB1C3 with USER endings. | ||
| + | |||
| + | Small gel: | ||
| + | * 1 kb ladder | ||
| + | * 2%, pur | ||
| + | * 4%, pur | ||
| + | * 5%, pur | ||
| + | * 4%, 1uL, pur | ||
| + | * 4%, 2uL, pur | ||
| + | * 1M, pur | ||
| + | * 2%, HQ | ||
| + | * 4%, HQ | ||
| + | * 5%, HQ | ||
| + | * 4%, 1uL, HQ | ||
| + | * 4%, 2uL, HQ | ||
| + | * 1M HQ | ||
| + | * 1 kb ladder (ladder was almost empty so I put another one in the next lane) | ||
| + | * 1 kb ladder | ||
| + | |||
| + | big gel (samples from touch down PCR): | ||
| + | |||
| + | * 1 kb ladder | ||
| + | * GC, HQ | ||
| + | * 2% HQ | ||
| + | * 4% HQ | ||
| + | * 5% HQ | ||
| + | * 4%, 1uL, HQ | ||
| + | * 4%, 2uL, HQ | ||
| + | * 1M HQ | ||
| + | * GC pur | ||
| + | * 2%, pur | ||
| + | * 4%, pur | ||
| + | * 5%, pur | ||
| + | * 4%, 1uL, pur | ||
| + | * 4%, 2uL, pur | ||
| + | * 1M pur | ||
| + | * neg | ||
| + | * 1 kb ladder | ||
==Conclusion== | ==Conclusion== | ||
Revision as of 11:20, 29 August 2013
29 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Results
Gel pictures
Ran gels on yesterday's PCRs to create psB1C3 with USER endings.
Small gel:
- 1 kb ladder
- 2%, pur
- 4%, pur
- 5%, pur
- 4%, 1uL, pur
- 4%, 2uL, pur
- 1M, pur
- 2%, HQ
- 4%, HQ
- 5%, HQ
- 4%, 1uL, HQ
- 4%, 2uL, HQ
- 1M HQ
- 1 kb ladder (ladder was almost empty so I put another one in the next lane)
- 1 kb ladder
big gel (samples from touch down PCR):
- 1 kb ladder
- GC, HQ
- 2% HQ
- 4% HQ
- 5% HQ
- 4%, 1uL, HQ
- 4%, 2uL, HQ
- 1M HQ
- GC pur
- 2%, pur
- 4%, pur
- 5%, pur
- 4%, 1uL, pur
- 4%, 2uL, pur
- 1M pur
- neg
- 1 kb ladder
Conclusion
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