lab 208

Main purpose

Who was in the lab

Procedure

Results

Gel pictures

Ran gels on yesterday's PCRs to create psB1C3 with USER endings.

Small gel:

• 1 kb ladder
• 2%, pur
• 4%, pur
• 5%, pur
• 4%, 1uL, pur
• 4%, 2uL, pur
• 1M, pur
• 2%, HQ
• 4%, HQ
• 5%, HQ
• 4%, 1uL, HQ
• 4%, 2uL, HQ
• 1M HQ
• 1 kb ladder (ladder was almost empty so I put another one in the next lane)
• 1 kb ladder

big gel (samples from touch down PCR):

• 1 kb ladder
• GC, HQ
• 2% HQ
• 4% HQ
• 5% HQ
• 4%, 1uL, HQ
• 4%, 2uL, HQ
• 1M HQ
• GC pur
• 2%, pur
• 4%, pur
• 5%, pur
• 4%, 1uL, pur
• 4%, 2uL, pur
• 1M pur
• neg
• 1 kb ladder

Conclusion

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