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Team:DTU-Denmark/Notebook/30 August 2013
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==Main purpose== | ==Main purpose== | ||
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==Results== | ==Results== | ||
<hr/> | <hr/> | ||
| + | |||
| + | ===Gel=== | ||
| + | |||
| + | * 1 kb ladder | ||
| + | * psB1C3 for Nir, 2% DMSO | ||
| + | * psB1C3 for Nir, 2% DMSO, 1 uL 50mM MgCl2 | ||
| + | * psB1C3 for Nir, 4% DMSO | ||
| + | * psB1C3 for Nir, 4% DMSO, 1 uL 50mM MgCl2 | ||
| + | * psB1C3 for Nir, 1M Betaine | ||
| + | * purification of psB1C3 USER fragment to make Biobricks (escaped from well -> Ethanol?) | ||
| + | * purification of HAO fragment (escaped from well -> Ethanol?) | ||
| + | * purification of AMO fragment (escaped from well -> Ethanol?) | ||
| + | * purification of psB1C3 USER fragment to make Biobricks (used more loading buffer) | ||
| + | * purification of HAO fragment (used more loading buffer) | ||
| + | * purification of AMO fragment (used more loading buffer) | ||
| + | * purification of pZA21::ara(tight) | ||
| + | * purification of pZA21::ara(tight) with endings for Nir | ||
| + | * 1 kb ladder | ||
==Conclusion== | ==Conclusion== | ||
Revision as of 14:43, 30 August 2013
30 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Procedure
Results
Gel
- 1 kb ladder
- psB1C3 for Nir, 2% DMSO
- psB1C3 for Nir, 2% DMSO, 1 uL 50mM MgCl2
- psB1C3 for Nir, 4% DMSO
- psB1C3 for Nir, 4% DMSO, 1 uL 50mM MgCl2
- psB1C3 for Nir, 1M Betaine
- purification of psB1C3 USER fragment to make Biobricks (escaped from well -> Ethanol?)
- purification of HAO fragment (escaped from well -> Ethanol?)
- purification of AMO fragment (escaped from well -> Ethanol?)
- purification of psB1C3 USER fragment to make Biobricks (used more loading buffer)
- purification of HAO fragment (used more loading buffer)
- purification of AMO fragment (used more loading buffer)
- purification of pZA21::ara(tight)
- purification of pZA21::ara(tight) with endings for Nir
- 1 kb ladder
Conclusion
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