Team:DTU-Denmark/Notebook/31 July 2013
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===USER=== | ===USER=== | ||
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+ | USER reaction to integrate Biobrick K808000 (AraBAD promoter system) in pZE21 containing either GFP SF or RFP. Did doubles for both with 7 uL of AraBAD and 15 uL to compare. Standard procedure. | ||
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+ | ===PCR=== | ||
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+ | Performed PCR on lambda phage as a test of polymerase efficiency. (PHUSION polymerase test kit) | ||
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+ | Some PCR with HAO and AMO. | ||
==Results== | ==Results== |
Revision as of 14:12, 31 July 2013
31 July 2013
Contents |
lab 208
Main purpose
- USER reaction to integrate Biobrick K808000 (AraBAD promoter system) in pZE21 containing fluorescence markers
- PCR
Who was in the lab
Kristian, Gosia, Julia, Henrike
Procedure
USER
USER reaction to integrate Biobrick K808000 (AraBAD promoter system) in pZE21 containing either GFP SF or RFP. Did doubles for both with 7 uL of AraBAD and 15 uL to compare. Standard procedure.
PCR
Performed PCR on lambda phage as a test of polymerase efficiency. (PHUSION polymerase test kit)
Some PCR with HAO and AMO.
Results
Gel on todays PCR
- 1kb ladder
- HAO col
- HAO col
- HAO par
- HAO par
- AMO col
- AMO col
- AMO par
- AMO par
- neg
- 1 kb ladder
- ...
Conclusion
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