lab 208

Main purpose

• USER reaction to integrate Biobrick K808000 (AraBAD promoter system) in pZE21 containing fluorescence markers
• PCR

Who was in the lab

Kristian, Gosia, Julia, Henrike

Procedure

USER

USER reaction to integrate Biobrick K808000 (AraBAD promoter system) in pZE21 containing either GFP SF or RFP. Did doubles for both with 7 uL of AraBAD and 15 uL to compare. Standard procedure.

PCR

Performed PCR on lambda phage as a test of polymerase efficiency. (PHUSION polymerase test kit)

Some PCR with HAO and AMO.

Results

Gel on todays PCR

• 1kb ladder
• HAO col
• HAO col
• HAO par
• HAO par
• AMO col
• AMO col
• AMO par
• AMO par
• neg
• 1 kb ladder
• polymerase test with PHUSION
• polymerase test with PHUSION
• polymerase test with X7
• polymerase test with X7

Conclusion

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