Team:DTU-Denmark/Notebook/3 August 2013

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==Who was in the lab==
==Who was in the lab==
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Kristian, Henrike
 
==Procedure==
==Procedure==

Revision as of 14:16, 3 August 2013

3 August 2013

Contents

lab 208


Main purpose


Who was in the lab


Procedure


Gel electrophoresis

Run 1% agarose gel on overnight PCR products.

Miniprep

Isolate plasmids from samples Sec2, cycAX, TAT 3-1a, TAT 3-2, TAT 2-1 using ethanol precipitation.

Results


Gels

1% agarose gel

  • 1kb ladder
  • Nir1 high
  • Nir1 high
  • Nir1 low
  • Nir1 low
  • broad band ladder
  • 43
  • 43
  • Nir2 GC
  • Nir2 GC
  • neg for Nir
  • AMO
  • AMO
  • neg for AMO
  • 1 kb ladder

2013-08-03 nir amo2.jpg

Conclusion


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