Team:DTU-Denmark/Notebook/4 July 2013
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==main purpose== | ==main purpose== | ||
- | We want to view our cells throught a | + | We want to view our cells throught a fluorescent microscope, in order to see if they have expressed GFP and RPF. |
Hopefully we will see GFP in the periplasm and RFP inside the cell. | Hopefully we will see GFP in the periplasm and RFP inside the cell. | ||
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+ | Setup an overnight culture of the 2 successful transformants so we can both MidiPrep and isolate periplasmic proteins tomorrow. | ||
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+ | ==Procedure== | ||
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==Results== | ==Results== | ||
We managed to see expressed GFP and RFP | We managed to see expressed GFP and RFP |
Revision as of 14:55, 4 July 2013
Contents |
301
main purpose
We want to view our cells throught a fluorescent microscope, in order to see if they have expressed GFP and RPF.
Hopefully we will see GFP in the periplasm and RFP inside the cell.
Setup an overnight culture of the 2 successful transformants so we can both MidiPrep and isolate periplasmic proteins tomorrow.
Procedure
Results
We managed to see expressed GFP and RFP