Team:DTU-Denmark/Notebook/4 September 2013
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+ | ===Colony PCR to confirm AMO and cycAX inserts in our Biobricks=== | ||
+ | |||
+ | Used Q5 master mix, 50 uL. | ||
+ | |||
+ | {| class="wikitable" style="text-align: right" | ||
+ | ! compound !! volume (uL) | ||
+ | |- | ||
+ | | Q5 || 25 | ||
+ | |- | ||
+ | | FW || 3 | ||
+ | |- | ||
+ | | RV || 3 | ||
+ | |- | ||
+ | | template || 1 | ||
+ | |- | ||
+ | | MQ || 18 | ||
+ | |- | ||
+ | |} | ||
+ | |||
+ | Following primer pairs: | ||
+ | * FW_2_AMO_Seq, RV_3_AMO_Seq, - 63C - expected fragment length: 650bp | ||
+ | * FW_1_cyc_Seq, RV_2_cyc_Seq - 61C - expected fragment length: 649bp | ||
+ | |||
+ | Program: | ||
+ | {| class="wikitable" style="text-align: right" | ||
+ | ! temperature !! time !! cycles | ||
+ | |- | ||
+ | | 98C || 10:00 || - | ||
+ | |- | ||
+ | | 98C || 0:10 || 35 | ||
+ | |- | ||
+ | | annealing temperature || 0:30 || 35 | ||
+ | |- | ||
+ | | 72C || 0:30 || 35 | ||
+ | |- | ||
+ | | 72C || 5:00 || - | ||
+ | |- | ||
+ | | 10C || hold || - | ||
+ | |- | ||
+ | |} | ||
==Results== | ==Results== |
Revision as of 11:40, 4 September 2013
4 September 2013
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Contents |
Lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR to confirm AMO and cycAX inserts in our Biobricks
Used Q5 master mix, 50 uL.
compound | volume (uL) |
---|---|
Q5 | 25 |
FW | 3 |
RV | 3 |
template | 1 |
MQ | 18 |
Following primer pairs:
- FW_2_AMO_Seq, RV_3_AMO_Seq, - 63C - expected fragment length: 650bp
- FW_1_cyc_Seq, RV_2_cyc_Seq - 61C - expected fragment length: 649bp
Program:
temperature | time | cycles |
---|---|---|
98C | 10:00 | - |
98C | 0:10 | 35 |
annealing temperature | 0:30 | 35 |
72C | 0:30 | 35 |
72C | 5:00 | - |
10C | hold | - |
Results
Conclusion
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