Team:DTU-Denmark/Notebook/4 September 2013

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===Colony PCR to confirm AMO and cycAX inserts in our Biobricks===
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Used Q5 master mix, 50 uL.
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{| class="wikitable" style="text-align: right"
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! compound !! volume (uL)
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|-
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| Q5 || 25
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|-
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| FW || 3
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|-
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| RV || 3
 +
|-
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| template || 1
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|-
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| MQ || 18
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|-
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|}
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 +
Following primer pairs:
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* FW_2_AMO_Seq, RV_3_AMO_Seq, - 63C - expected fragment length: 650bp
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* FW_1_cyc_Seq, RV_2_cyc_Seq - 61C - expected fragment length: 649bp
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Program:
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{| class="wikitable" style="text-align: right"
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! temperature !! time !! cycles
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|-
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|  98C || 10:00 || -
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|-
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|  98C || 0:10 || 35
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|-
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|  annealing temperature || 0:30 || 35
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|-
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|  72C || 0:30 || 35
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|-
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|  72C || 5:00 || -
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|-
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|  10C || hold || -
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|-
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|}
==Results==
==Results==

Revision as of 11:40, 4 September 2013

4 September 2013

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Contents

Lab 208

Main purpose


Who was in the lab


Procedure

Colony PCR to confirm AMO and cycAX inserts in our Biobricks

Used Q5 master mix, 50 uL.

compound volume (uL)
Q5 25
FW 3
RV 3
template 1
MQ 18

Following primer pairs:

  • FW_2_AMO_Seq, RV_3_AMO_Seq, - 63C - expected fragment length: 650bp
  • FW_1_cyc_Seq, RV_2_cyc_Seq - 61C - expected fragment length: 649bp

Program:

temperature time cycles
98C 10:00 -
98C 0:10 35
annealing temperature 0:30 35
72C 0:30 35
72C 5:00 -
10C hold -

Results

Conclusion


Navigate to the Previous or the Next Entry

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