"
Team:DTU-Denmark/Notebook/4 September 2013
From 2013.igem.org
(Difference between revisions)
(→Procedure) |
|||
| Line 6: | Line 6: | ||
==Main purpose== | ==Main purpose== | ||
<hr/> | <hr/> | ||
| - | + | *Colony PCR to confirm AMO and cycAX inserts in our Biobricks | |
==Who was in the lab== | ==Who was in the lab== | ||
Revision as of 08:54, 6 September 2013
4 September 2013
Navigate to the Previous or the Next Entry
Contents |
Lab 208
Main purpose
- Colony PCR to confirm AMO and cycAX inserts in our Biobricks
Who was in the lab
Procedure
Colony PCR to confirm AMO and cycAX inserts in our Biobricks
Used Q5 master mix, 50 uL.
| compound | volume (uL) |
|---|---|
| Q5 | 25 |
| FW | 3 |
| RV | 3 |
| template | 1 |
| MQ | 18 |
Following primer pairs:
- FW_2_AMO_Seq, RV_3_AMO_Seq, - 63C - expected fragment length: 650bp
- FW_1_cyc_Seq, RV_2_cyc_Seq - 61C - expected fragment length: 649bp
Program:
| temperature | time | cycles |
|---|---|---|
| 98C | 10:00 | - |
| 98C | 0:10 | 35 |
| annealing temperature | 0:30 | 35 |
| 72C | 0:30 | 35 |
| 72C | 5:00 | - |
| 10C | hold | - |
Results
Conclusion
Navigate to the Previous or the Next Entry
