Team:DTU-Denmark/Notebook/4 September 2013
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4 September 2013
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Contents |
Lab 208
Main purpose
Who was in the lab
Procedure
Colony PCR to confirm AMO and cycAX inserts in our Biobricks
Used Q5 master mix, 50 uL.
compound | volume (uL) |
---|---|
Q5 | 25 |
FW | 3 |
RV | 3 |
template | 1 |
MQ | 18 |
Following primer pairs:
- FW_2_AMO_Seq, RV_3_AMO_Seq, - 63C - expected fragment length: 650bp
- FW_1_cyc_Seq, RV_2_cyc_Seq - 61C - expected fragment length: 649bp
Program:
temperature | time | cycles |
---|---|---|
98C | 10:00 | - |
98C | 0:10 | 35 |
annealing temperature | 0:30 | 35 |
72C | 0:30 | 35 |
72C | 5:00 | - |
10C | hold | - |
Results
Conclusion
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