Team:DTU-Denmark/Notebook/5 September 2013

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(Procedure)
(Lab 208)
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==Main purpose==
==Main purpose==
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PCR for HAO with USER endings
+
PCR for HAO with USER endings after purification
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+
==Who was in the lab==
==Who was in the lab==
Henrike, Ariadni
Henrike, Ariadni
-
 
==Procedure==
==Procedure==

Revision as of 07:10, 6 September 2013

5 September 2013

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Contents

Lab 208


Main purpose

PCR for HAO with USER endings after purification

Who was in the lab

Henrike, Ariadni

Procedure

Template: HAO extraction fragment (purified from PCR run on 04.09) Two versions: one with 5% DMSO and one with 3% DMSO

PCR reaction master mix (x5)

compound volume (uL)
dNTPs 1
HF 10
polymerase x7 0.5
FW 3
RV 3
template 1
MQ 29
DMSO 1.5
  • Add 49 ul of the master mix, and
  • Add 1 ul DMSO (for the 5%) or 1 ul of MQ (for the 3%)

Results


Conclusion



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